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quantikine human upar immunoassay elisa kit  (R&D Systems)


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    R&D Systems quantikine human upar immunoassay elisa kit
    Quantikine Human Upar Immunoassay Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantikine+human+upar+immunoassay/Human+uPAR+Quantikine+ELISA+Kit/pm38857636-242-10-16
    Average 94 stars, based on 73 article reviews
    quantikine human upar immunoassay elisa kit - by Bioz Stars, 2026-10
    94/100 stars

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    Concentration Assay:

    Article Title: Soluble Urokinase Receptor Levels in Secondary Focal Segmental Glomerulosclerosis
    Article Snippet: .. We detected the concentration of plasma and urinary suPAR using the Quantikine Human uPAR Immunoassay (R&D Systems, Minneapolis, MN, USA) following the manufacturer's protocol. .. In brief, the assay involved 5 steps: (1) 96-well polystyrene microplates were precoated with mouse monoclonal antibody against uPAR; (2) both plasma and urine samples were diluted 1:5 and added to each well and incubated for 2 h at room temperature; (3) after incubation and washing, horseradish peroxidase-conjugated polyclonal antibodies against uPAR were added and there was incubation for 2 h at room temperature; (4) after washing, substrate solution was added to each well and the contents were incubated for 30 min at room temperature, and protected from light; and (5) a stop solution was added to each well and absorbance was recorded using an enzyme-linked immunosorbent assay (ELISA) reader at 450/570 nm.

    Article Title: Patients With Combined Membranous Nephropathy and Focal Segmental Glomerulosclerosis Have Comparable Clinical and Autoantibody Profiles With Primary Membranous Nephropathy
    Article Snippet: .. The concentration of plasma and urinary suPAR was detected with Quantikine Human uPAR Immunoassay (R&D Systems, Minneapolis, MN), according to the manufacturer’s instruction as previously reported.20,21 .. Statistical analysis was performed using the SPSS statistical software package, version 13.0 (SPSS Inc., Chicago, IL).

    Article Title: Primary and Recurrent Focal Segmental Glomerulosclerosis Closely Link to Serum Soluble Urokinase-type Plasminogen Activator Receptor Levels.
    Article Snippet: Background.. Serum soluble urokinase-type plasminogen activator receptor (suPAR) is implicated in the pathogenesis of native and recurrent focal segmental glomerulosclerosis (FSGS).. It is elevated in two-thirds of subjects with primary FSGS, but not in people with other glomerular diseases that can differentiate FSGS and other glomerular diseases.

    Article Title: MeSsAGe risk score: tool for renal biopsy decision in steroid-dependent nephrotic syndrome.
    Article Snippet: .. Plasma suPAR concentration was determined using Quantikine Human uPAR Immunoassay (R&D Systems, Inc, Minneapolis, MN) according to the manufacturer’s protocol. ..

    Clinical Proteomics:

    Article Title: Soluble Urokinase Receptor Levels in Secondary Focal Segmental Glomerulosclerosis
    Article Snippet: .. We detected the concentration of plasma and urinary suPAR using the Quantikine Human uPAR Immunoassay (R&D Systems, Minneapolis, MN, USA) following the manufacturer's protocol. .. In brief, the assay involved 5 steps: (1) 96-well polystyrene microplates were precoated with mouse monoclonal antibody against uPAR; (2) both plasma and urine samples were diluted 1:5 and added to each well and incubated for 2 h at room temperature; (3) after incubation and washing, horseradish peroxidase-conjugated polyclonal antibodies against uPAR were added and there was incubation for 2 h at room temperature; (4) after washing, substrate solution was added to each well and the contents were incubated for 30 min at room temperature, and protected from light; and (5) a stop solution was added to each well and absorbance was recorded using an enzyme-linked immunosorbent assay (ELISA) reader at 450/570 nm.

    Article Title: Patients With Combined Membranous Nephropathy and Focal Segmental Glomerulosclerosis Have Comparable Clinical and Autoantibody Profiles With Primary Membranous Nephropathy
    Article Snippet: .. The concentration of plasma and urinary suPAR was detected with Quantikine Human uPAR Immunoassay (R&D Systems, Minneapolis, MN), according to the manufacturer’s instruction as previously reported.20,21 .. Statistical analysis was performed using the SPSS statistical software package, version 13.0 (SPSS Inc., Chicago, IL).

    Article Title: Plasma Soluble Urokinase Receptor Level Is Correlated with Podocytes Damage in Patients with IgA Nephropathy
    Article Snippet: .. Plasma suPAR was quantified by the Quantikine Human uPAR Immunoassay (R&D Systems), according to the manufacturer’s protocol. ..

    Article Title: MeSsAGe risk score: tool for renal biopsy decision in steroid-dependent nephrotic syndrome.
    Article Snippet: .. Plasma suPAR concentration was determined using Quantikine Human uPAR Immunoassay (R&D Systems, Inc, Minneapolis, MN) according to the manufacturer’s protocol. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Expression of Urokinase-type Plasminogen Activator Receptor and its Soluble Form in Type 2 Diabetic Kidney Disease.
    Article Snippet: Expression of Urokinase-type Plasminogen Activator Receptor and its Soluble Form in Type 2 Diabetic Kidney Disease Yujing Zhou,* Jianmin Ren,* Peng Li, Rong Ma, Mengkun Zhou, Ningxin Zhang, Xiangguo Kong, Zhao Hu, and Xiaoyan Xiao Department of Endocrinology, Qilu Hospital of Shandong University, Jinan, China Institute of Endocrinology and Metabolism, Shandong University, Jinan, China Key Laboratory of Endocrinology and Metabolism, Shandong Province in Medicine and Health, Jinan, China Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai, China Department of Nephrology, Yuhuangding Hospital Affiliated to Qingdao University, Yantai, China Department of Nephrology, Qilu Hospital of Shandong University, Jinan, China

    Article Title: Soluble Urokinase Plasminogen Activator Receptor as a Diagnostic and Prognostic Biomarker in Cardiac Disease
    Article Snippet: .. Two different kits are most commonly used, suPARnostic ® ELISA (Virogates) and Quantikine Human uPAR Immunoassay (R&D Systems), both of which are highly accurate with a threshold of 100 pg/mL for detection. ..

    other:

    Article Title: Soluble Urokinase Receptor and Chronic Kidney Disease
    Article Snippet: We then measured suPAR using a different assay (Quantikine Human uPAR Immunoassay [R&D Systems]) in a separate replication cohort of 347 women enrolled in the WIHS (mean age, 40 years; 62% of black race; 39% with positivity for the human immunodeficiency virus) ( Table S6 in the Supplementary Appendix ) for whom follow-up data on the eGFR were available (median, 26 measurements over a period of ≥10 years).



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    Image Search Results


    ET-1 promotes invasion of MCs and transmesothelial migration of SOC cells. (A) Transwell invasion assays of MCs transfected with si-SCR or si-ARRB1 and stimulated with ET-1 and/or BQ123+BQ788 for 12 h. Histograms, the mean ± SD, n = 6. One-way ANOVA. (B) MMP-7 or (C) uPAR secretion evaluated by ELISA in CM from MCs at indicated times. Histograms, the mean ± SD, n = 2. One-way ANOVA. (D) Transmesothelial migration assay of OVCAR3 cells seeded into the upper chamber with a monolayer of MCs on a fibronectin-coated membrane and treated with ET-1 and/or BQ123+BQ788 for 24 h. Histograms, the mean ± SD, n = 6. One-way ANOVA.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Ovarian Cancer-Driven Mesothelial-to-Mesenchymal Transition is Triggered by the Endothelin-1/β-arr1 Axis

    doi: 10.3389/fcell.2021.764375

    Figure Lengend Snippet: ET-1 promotes invasion of MCs and transmesothelial migration of SOC cells. (A) Transwell invasion assays of MCs transfected with si-SCR or si-ARRB1 and stimulated with ET-1 and/or BQ123+BQ788 for 12 h. Histograms, the mean ± SD, n = 6. One-way ANOVA. (B) MMP-7 or (C) uPAR secretion evaluated by ELISA in CM from MCs at indicated times. Histograms, the mean ± SD, n = 2. One-way ANOVA. (D) Transmesothelial migration assay of OVCAR3 cells seeded into the upper chamber with a monolayer of MCs on a fibronectin-coated membrane and treated with ET-1 and/or BQ123+BQ788 for 24 h. Histograms, the mean ± SD, n = 6. One-way ANOVA.

    Article Snippet: Following the manufacturer’s protocols, uPAR, MMP-7 and ET-1 secretion were evaluated by using a Human uPAR Immunoassay (Cat# DUP00 R&D), Human Total MMP-7 Quantikine ELISA Kit (Cat# DMP700, R&D), and ET-1 Human Endothelin 1 ELISA Kit (Car# CEK1146, Cohesion Biosciences), respectively, following manufacturer’s instructions.

    Techniques: Migration, Transfection, Enzyme-linked Immunosorbent Assay, Membrane

    Comparison of suPAR levels on ELISA vs MSD platforms. The left panel shows the distribution of suPAR measurements from the two suPAR assays. The right panel shows the distribution of the individual differences in measurements between the two assays. Abbreviations: ELISA, enzyme-linked immunosorbent assay (Quantikine); MSD, Meso Scale Discovery; suPAR, soluble urokinase plasminogen activator receptor.

    Journal: Kidney Medicine

    Article Title: Variability in CKD Biomarker Studies: Soluble Urokinase Plasminogen Activator Receptor (suPAR) and Kidney Disease Progression in the Chronic Kidney Disease in Children (CKiD) Study

    doi: 10.1016/j.xkme.2021.04.007

    Figure Lengend Snippet: Comparison of suPAR levels on ELISA vs MSD platforms. The left panel shows the distribution of suPAR measurements from the two suPAR assays. The right panel shows the distribution of the individual differences in measurements between the two assays. Abbreviations: ELISA, enzyme-linked immunosorbent assay (Quantikine); MSD, Meso Scale Discovery; suPAR, soluble urokinase plasminogen activator receptor.

    Article Snippet: For study A, suPAR was measured from plasma collected at the 6-month CKiD visit in duplicate using Quantikine Enzyme-Linked Immunosorbent Assay (ELISA) immunoassay (catalog #DUP00; R&D Systems).

    Techniques: Comparison, Enzyme-linked Immunosorbent Assay

    Agreement of suPAR measurements from 2 assays. The left panel shows the deviation of agreement from the line of identity on the natural scale showing both a shift of the central tendency and a slope change. The right panel shows the results from Bland Altman analysis after natural log transformation to normalize distributions showing a bias, difference in the spread of the data and modest linear correlation. Study A measurements were performed with ELISA, and study B measurements were performed with MSD. The bias was estimated as the mean of the differences in measurements. The Pearson correlation and ratio of standard deviations are also shown. Abbreviations: ELISA, enzyme-linked immunosorbent assay (Quantikine); MSD, Meso Scale Discovery; suPAR, soluble urokinase plasminogen activator receptor.

    Journal: Kidney Medicine

    Article Title: Variability in CKD Biomarker Studies: Soluble Urokinase Plasminogen Activator Receptor (suPAR) and Kidney Disease Progression in the Chronic Kidney Disease in Children (CKiD) Study

    doi: 10.1016/j.xkme.2021.04.007

    Figure Lengend Snippet: Agreement of suPAR measurements from 2 assays. The left panel shows the deviation of agreement from the line of identity on the natural scale showing both a shift of the central tendency and a slope change. The right panel shows the results from Bland Altman analysis after natural log transformation to normalize distributions showing a bias, difference in the spread of the data and modest linear correlation. Study A measurements were performed with ELISA, and study B measurements were performed with MSD. The bias was estimated as the mean of the differences in measurements. The Pearson correlation and ratio of standard deviations are also shown. Abbreviations: ELISA, enzyme-linked immunosorbent assay (Quantikine); MSD, Meso Scale Discovery; suPAR, soluble urokinase plasminogen activator receptor.

    Article Snippet: For study A, suPAR was measured from plasma collected at the 6-month CKiD visit in duplicate using Quantikine Enzyme-Linked Immunosorbent Assay (ELISA) immunoassay (catalog #DUP00; R&D Systems).

    Techniques: Transformation Assay, Enzyme-linked Immunosorbent Assay

    Comparison of dendrograms from hierarchical clustering analysis using complete linkage. Each leaf or line corresponds to 1 observation. Observations that are similar to each other are combined (fused) as the dendrogram flows away from the center. The height of the fusion along the horizontal axis indicates the (dis)similarity between 2 observations. The farther away from the center the fusion occurs, the less similar the observations are. The dendrogram on the left shows relationships between participants’ given values of ELISA suPAR, BMI z score, log 2 (UPCR), age, eGFR, and BUN. The dendrogram on the right shows relationships between participants’ given values of MSD suPAR, BMI z score, log2(UPCR), age, eGFR, and BUN. Grey lines illustrate how individuals re-sort depending on whether ELISA or MSD is used for suPAR measurement. The quality of the alignment of the 2 trees is indicated by the entanglement. Entanglement is a measure between 1 (full entanglement) and 0 (no entanglement). A lower entanglement coefficient corresponds to a good alignment. Abbreviations: BMI, body mass index; eGFR, estimated glomerular filtration rate; ELISA, enzyme-linked immunosorbent assay (Quantikine); MSD, Meso Scale Discovery; BUN, blood urea nitrogen; suPAR, soluble urokinase plasminogen activator receptor; UPCR, urinary protein-creatinine ratio.

    Journal: Kidney Medicine

    Article Title: Variability in CKD Biomarker Studies: Soluble Urokinase Plasminogen Activator Receptor (suPAR) and Kidney Disease Progression in the Chronic Kidney Disease in Children (CKiD) Study

    doi: 10.1016/j.xkme.2021.04.007

    Figure Lengend Snippet: Comparison of dendrograms from hierarchical clustering analysis using complete linkage. Each leaf or line corresponds to 1 observation. Observations that are similar to each other are combined (fused) as the dendrogram flows away from the center. The height of the fusion along the horizontal axis indicates the (dis)similarity between 2 observations. The farther away from the center the fusion occurs, the less similar the observations are. The dendrogram on the left shows relationships between participants’ given values of ELISA suPAR, BMI z score, log 2 (UPCR), age, eGFR, and BUN. The dendrogram on the right shows relationships between participants’ given values of MSD suPAR, BMI z score, log2(UPCR), age, eGFR, and BUN. Grey lines illustrate how individuals re-sort depending on whether ELISA or MSD is used for suPAR measurement. The quality of the alignment of the 2 trees is indicated by the entanglement. Entanglement is a measure between 1 (full entanglement) and 0 (no entanglement). A lower entanglement coefficient corresponds to a good alignment. Abbreviations: BMI, body mass index; eGFR, estimated glomerular filtration rate; ELISA, enzyme-linked immunosorbent assay (Quantikine); MSD, Meso Scale Discovery; BUN, blood urea nitrogen; suPAR, soluble urokinase plasminogen activator receptor; UPCR, urinary protein-creatinine ratio.

    Article Snippet: For study A, suPAR was measured from plasma collected at the 6-month CKiD visit in duplicate using Quantikine Enzyme-Linked Immunosorbent Assay (ELISA) immunoassay (catalog #DUP00; R&D Systems).

    Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Filtration

    Probability of composite event of end-stage kidney disease or >50% decline in glomerular filtration rate based on quartile (Q) categories. Area of the diamond within each square represents magnitude of the risk of the composite event. Numerators are the number of events and denominators are the number of individuals in each cross category of ELISA and MSD quartile. Abbreviations: ELISA, enzyme-linked immunosorbent assay (Quantikine); MSD, Meso Scale Discovery.

    Journal: Kidney Medicine

    Article Title: Variability in CKD Biomarker Studies: Soluble Urokinase Plasminogen Activator Receptor (suPAR) and Kidney Disease Progression in the Chronic Kidney Disease in Children (CKiD) Study

    doi: 10.1016/j.xkme.2021.04.007

    Figure Lengend Snippet: Probability of composite event of end-stage kidney disease or >50% decline in glomerular filtration rate based on quartile (Q) categories. Area of the diamond within each square represents magnitude of the risk of the composite event. Numerators are the number of events and denominators are the number of individuals in each cross category of ELISA and MSD quartile. Abbreviations: ELISA, enzyme-linked immunosorbent assay (Quantikine); MSD, Meso Scale Discovery.

    Article Snippet: For study A, suPAR was measured from plasma collected at the 6-month CKiD visit in duplicate using Quantikine Enzyme-Linked Immunosorbent Assay (ELISA) immunoassay (catalog #DUP00; R&D Systems).

    Techniques: Filtration, Enzyme-linked Immunosorbent Assay

    The results of laboratory test performed in the studied patients with acute pancreatitis on the day of admission. Data are shown as median (lower; upper quartile).

    Journal: Journal of Clinical Medicine

    Article Title: The Marker of Tubular Injury, Kidney Injury Molecule-1 (KIM-1), in Acute Kidney Injury Complicating Acute Pancreatitis: A Preliminary Study

    doi: 10.3390/jcm9051463

    Figure Lengend Snippet: The results of laboratory test performed in the studied patients with acute pancreatitis on the day of admission. Data are shown as median (lower; upper quartile).

    Article Snippet: Serum concentrations of uPAR were measured using Quantikine ELISA Human uPAR Immunoassay (R & D Systems, McKinley Place, MN, USA).

    Techniques:

    Correlations between KIM-1 and the selected laboratory results on days 1, 2, and 3 of the study in the studied patients with acute pancreatitis.

    Journal: Journal of Clinical Medicine

    Article Title: The Marker of Tubular Injury, Kidney Injury Molecule-1 (KIM-1), in Acute Kidney Injury Complicating Acute Pancreatitis: A Preliminary Study

    doi: 10.3390/jcm9051463

    Figure Lengend Snippet: Correlations between KIM-1 and the selected laboratory results on days 1, 2, and 3 of the study in the studied patients with acute pancreatitis.

    Article Snippet: Serum concentrations of uPAR were measured using Quantikine ELISA Human uPAR Immunoassay (R & D Systems, McKinley Place, MN, USA).

    Techniques: